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synaptosome isolation kit  (Invent Biotechnologies)


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    Invent Biotechnologies synaptosome isolation kit
    (A) Ibogalogs reduce the TBARS level in hippocampal (HPC) and frontal cortex (FC) synaptosomal fractions compared to AAPH-treatment, one-way ANOVA followed by Tukey’s posthoc test ( n = 4). (B) Western blot verification of synaptophysin levels (synaptosomal marker) in <t>synaptosomes</t> prepared from rat HPC and FC. The molecular weight of the loaded protein (5 μg/lane) was verified with PageRuler Plus Prestained Protein Ladder.
    Synaptosome Isolation Kit, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/synaptosome+isolation+kit/pmc13047532-238-7-10?v=Invent+Biotechnologies
    Average 94 stars, based on 12 article reviews
    synaptosome isolation kit - by Bioz Stars, 2026-07
    94/100 stars

    Images

    1) Product Images from "Structure-Dependent Antioxidant Activity of Ibogalogs: Impact of Methoxy Group Position on the Protective Activity in Model and Synaptosomal Lipid Membranes"

    Article Title: Structure-Dependent Antioxidant Activity of Ibogalogs: Impact of Methoxy Group Position on the Protective Activity in Model and Synaptosomal Lipid Membranes

    Journal: ACS Chemical Neuroscience

    doi: 10.1021/acschemneuro.6c00087

    (A) Ibogalogs reduce the TBARS level in hippocampal (HPC) and frontal cortex (FC) synaptosomal fractions compared to AAPH-treatment, one-way ANOVA followed by Tukey’s posthoc test ( n = 4). (B) Western blot verification of synaptophysin levels (synaptosomal marker) in synaptosomes prepared from rat HPC and FC. The molecular weight of the loaded protein (5 μg/lane) was verified with PageRuler Plus Prestained Protein Ladder.
    Figure Legend Snippet: (A) Ibogalogs reduce the TBARS level in hippocampal (HPC) and frontal cortex (FC) synaptosomal fractions compared to AAPH-treatment, one-way ANOVA followed by Tukey’s posthoc test ( n = 4). (B) Western blot verification of synaptophysin levels (synaptosomal marker) in synaptosomes prepared from rat HPC and FC. The molecular weight of the loaded protein (5 μg/lane) was verified with PageRuler Plus Prestained Protein Ladder.

    Techniques Used: Western Blot, Marker, Molecular Weight



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    (A) Ibogalogs reduce the TBARS level in hippocampal (HPC) and frontal cortex (FC) synaptosomal fractions compared to AAPH-treatment, one-way ANOVA followed by Tukey’s posthoc test ( n = 4). (B) Western blot verification of synaptophysin levels (synaptosomal marker) in <t>synaptosomes</t> prepared from rat HPC and FC. The molecular weight of the loaded protein (5 μg/lane) was verified with PageRuler Plus Prestained Protein Ladder.
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    (A) Ibogalogs reduce the TBARS level in hippocampal (HPC) and frontal cortex (FC) synaptosomal fractions compared to AAPH-treatment, one-way ANOVA followed by Tukey’s posthoc test ( n = 4). (B) Western blot verification of synaptophysin levels (synaptosomal marker) in <t>synaptosomes</t> prepared from rat HPC and FC. The molecular weight of the loaded protein (5 μg/lane) was verified with PageRuler Plus Prestained Protein Ladder.
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    VMAT2 expression and protection against cytotoxicity by LMK‐235. (A) LMK‐235 induces VMAT2 gene expression in SH‐SY5Y cells in a concentration‐dependent manner. (B) The concentration of dopamine (DA) in the <t>synaptosomes</t> of SH‐SY5Y cells following LMK‐235 treatment. (C) Inhibition of cytotoxicity induced by 1 mM MPP + and/or 10 μM free DA (DA) with LMK‐235 treatment at various concentrations. (D) Inhibition of cytotoxicity induced by 500 μM 6‐OHDA and/or 10 μM DA with LMK‐235 treatment at different concentrations. Data are expressed as mean ± SEM. Statistical significance is indicated by * p < 0.05, ** p < 0.01 (Student's t test). For panels A and B, comparisons are made between the control and each concentration of LMK‐235. For panels C and D, comparisons are made between the MPP + + DA group and each concentration of LMK‐235. Symbols † p < 0.05 and †† p < 0.01 denote statistical significance compared to the MPP + + DA group under each condition. 6‐OHDA, VMAT2.
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    VMAT2 expression and protection against cytotoxicity by LMK‐235. (A) LMK‐235 induces VMAT2 gene expression in SH‐SY5Y cells in a concentration‐dependent manner. (B) The concentration of dopamine (DA) in the <t>synaptosomes</t> of SH‐SY5Y cells following LMK‐235 treatment. (C) Inhibition of cytotoxicity induced by 1 mM MPP + and/or 10 μM free DA (DA) with LMK‐235 treatment at various concentrations. (D) Inhibition of cytotoxicity induced by 500 μM 6‐OHDA and/or 10 μM DA with LMK‐235 treatment at different concentrations. Data are expressed as mean ± SEM. Statistical significance is indicated by * p < 0.05, ** p < 0.01 (Student's t test). For panels A and B, comparisons are made between the control and each concentration of LMK‐235. For panels C and D, comparisons are made between the MPP + + DA group and each concentration of LMK‐235. Symbols † p < 0.05 and †† p < 0.01 denote statistical significance compared to the MPP + + DA group under each condition. 6‐OHDA, VMAT2.
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    Image Search Results


    (A) Ibogalogs reduce the TBARS level in hippocampal (HPC) and frontal cortex (FC) synaptosomal fractions compared to AAPH-treatment, one-way ANOVA followed by Tukey’s posthoc test ( n = 4). (B) Western blot verification of synaptophysin levels (synaptosomal marker) in synaptosomes prepared from rat HPC and FC. The molecular weight of the loaded protein (5 μg/lane) was verified with PageRuler Plus Prestained Protein Ladder.

    Journal: ACS Chemical Neuroscience

    Article Title: Structure-Dependent Antioxidant Activity of Ibogalogs: Impact of Methoxy Group Position on the Protective Activity in Model and Synaptosomal Lipid Membranes

    doi: 10.1021/acschemneuro.6c00087

    Figure Lengend Snippet: (A) Ibogalogs reduce the TBARS level in hippocampal (HPC) and frontal cortex (FC) synaptosomal fractions compared to AAPH-treatment, one-way ANOVA followed by Tukey’s posthoc test ( n = 4). (B) Western blot verification of synaptophysin levels (synaptosomal marker) in synaptosomes prepared from rat HPC and FC. The molecular weight of the loaded protein (5 μg/lane) was verified with PageRuler Plus Prestained Protein Ladder.

    Article Snippet: Tissues were immediately processed using the Minute Synaptosome Isolation Kit (Invent Biotechnologies, Plymouth, MN, USA) for synaptosome fraction isolation.

    Techniques: Western Blot, Marker, Molecular Weight

    VMAT2 expression and protection against cytotoxicity by LMK‐235. (A) LMK‐235 induces VMAT2 gene expression in SH‐SY5Y cells in a concentration‐dependent manner. (B) The concentration of dopamine (DA) in the synaptosomes of SH‐SY5Y cells following LMK‐235 treatment. (C) Inhibition of cytotoxicity induced by 1 mM MPP + and/or 10 μM free DA (DA) with LMK‐235 treatment at various concentrations. (D) Inhibition of cytotoxicity induced by 500 μM 6‐OHDA and/or 10 μM DA with LMK‐235 treatment at different concentrations. Data are expressed as mean ± SEM. Statistical significance is indicated by * p < 0.05, ** p < 0.01 (Student's t test). For panels A and B, comparisons are made between the control and each concentration of LMK‐235. For panels C and D, comparisons are made between the MPP + + DA group and each concentration of LMK‐235. Symbols † p < 0.05 and †† p < 0.01 denote statistical significance compared to the MPP + + DA group under each condition. 6‐OHDA, VMAT2.

    Journal: Pharmacology Research & Perspectives

    Article Title: HDAC4 /5 Inhibitor, LMK ‐235 Improves Animal Voluntary Movement in MPTP ‐Induced Parkinson's Disease Model

    doi: 10.1002/prp2.70057

    Figure Lengend Snippet: VMAT2 expression and protection against cytotoxicity by LMK‐235. (A) LMK‐235 induces VMAT2 gene expression in SH‐SY5Y cells in a concentration‐dependent manner. (B) The concentration of dopamine (DA) in the synaptosomes of SH‐SY5Y cells following LMK‐235 treatment. (C) Inhibition of cytotoxicity induced by 1 mM MPP + and/or 10 μM free DA (DA) with LMK‐235 treatment at various concentrations. (D) Inhibition of cytotoxicity induced by 500 μM 6‐OHDA and/or 10 μM DA with LMK‐235 treatment at different concentrations. Data are expressed as mean ± SEM. Statistical significance is indicated by * p < 0.05, ** p < 0.01 (Student's t test). For panels A and B, comparisons are made between the control and each concentration of LMK‐235. For panels C and D, comparisons are made between the MPP + + DA group and each concentration of LMK‐235. Symbols † p < 0.05 and †† p < 0.01 denote statistical significance compared to the MPP + + DA group under each condition. 6‐OHDA, VMAT2.

    Article Snippet: Synaptosomes were isolated using a Minute Synaptosome Isolation Kit (SY‐052; Invitrogen, Waltham, MA, USA), and the DA concentration in the cytosol or synaptosomes was measured using a DA ELISA kit (ab285238; Abcam).

    Techniques: Expressing, Concentration Assay, Inhibition, Control